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| PMID |
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| TITLE |
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| Single cell transcriptomics reveals opioid usage evokes widespread suppression of antiviral gene program. |
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| ABSTRACT |
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| Chronic opioid usage not only causes addiction behavior through the central nervous system, but also modulates the peripheral immune system. However, how opioid impacts the immune system is still barely characterized systematically. In order to understand the immune modulatory effect of opioids in an unbiased way, here we perform single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells from opioid-dependent individuals and controls to show that chronic opioid usage evokes widespread suppression of antiviral gene program in naive monocytes, as well as in multiple immune cell types upon stimulation with the pathogen component lipopolysaccharide. Furthermore, scRNA-seq reveals the same phenomenon after a short in vitro morphine treatment. These findings indicate that both acute and chronic opioid exposure may be harmful to our immune system by suppressing the antiviral gene program. Our results suggest that further characterization of the immune modulatory effects of opioid is critical to ensure the safety of clinical opioids. |
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| DATE PUBLISHED |
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| HISTORY |
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| PUBSTATUS |
PUBSTATUSDATE |
| received |
2019/07/18 |
| accepted |
2020/04/19 |
| entrez |
2020/05/28 06:00 |
| pubmed |
2020/05/28 06:00 |
| medline |
2020/05/28 06:00 |
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| AUTHORS |
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| NAME |
COLLECTIVENAME |
LASTNAME |
FORENAME |
INITIALS |
AFFILIATION |
AFFILIATIONINFO |
| Karagiannis TT |
|
Karagiannis |
Tanya T |
TT |
|
Department of Biology, Boston University, 5 Cummington Mall, Boston, MA, 02215, USA. |
| Cleary JP Jr |
|
Cleary |
John P |
JP |
|
Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, 24 Cummington Mall, Boston, MA, 02215, USA. |
| Gok B |
|
Gok |
Busra |
B |
|
Program in Cell and Molecular Biology, Boston University, 24 Cummington Mall, Boston, MA, 02215, USA. |
| Henderson AJ |
|
Henderson |
Andrew J |
AJ |
|
Department of Medicine and Microbiology, Boston University School of Medicine, 650 Albany St, Boston, MA, 02215, USA. |
| Martin NG |
|
Martin |
Nicholas G |
NG |
|
QIMR Berghofer Medical Research Institute, Brisbane, QLD, 4006, Australia. |
| Yajima M |
|
Yajima |
Masanao |
M |
|
Department of Mathematics and Statistics, Boston University, 111 Cummington Mall, Boston, MA, 02215, USA. |
| Nelson EC |
|
Nelson |
Elliot C |
EC |
|
Department of Psychiatry, Washington University School of Medicine, 660S. Euclid Ave, St. Louis, MO, 63110, USA. |
| Cheng CS |
|
Cheng |
Christine S |
CS |
|
Program in Cell and Molecular Biology, Boston University, 24 Cummington Mall, Boston, MA, 02215, USA. chcheng@bu.edu. |
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| INVESTIGATORS |
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| JOURNAL |
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| VOLUME: 11 |
| ISSUE: 1 |
| TITLE: Nature communications |
| ISOABBREVIATION: Nat Commun |
| YEAR: 2020 |
| MONTH: May |
| DAY: 26 |
| MEDLINEDATE: |
| SEASON: |
| CITEDMEDIUM: Internet |
| ISSN: 2041-1723 |
| ISSNTYPE: Electronic |
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| MEDLINE JOURNAL |
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| MEDLINETA: Nat Commun |
| COUNTRY: England |
| ISSNLINKING: 2041-1723 |
| NLMUNIQUEID: 101528555 |
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| PUBLICATION TYPE |
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| PUBLICATIONTYPE TEXT |
| Journal Article |
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| COMMENTS AND CORRECTIONS |
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| GRANTS |
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| GRANTID |
AGENCY |
COUNTRY |
| R61DA047032 |
U.S. Department of Health & Human Services | NIH | National Institute on Drug Abuse (NIDA) |
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