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PMID |
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TITLE |
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Replicated effects of sex and genotype on gene expression in human lymphoblastoid cell lines. |
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ABSTRACT |
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The expression level for 15,887 transcripts in lymphoblastoid cell lines from 19 monozygotic twin pairs (10 male, 9 female) were analysed for the effects of genotype and sex. On an average, the effect of twin pairs explained 31% of the variance in normalized gene expression levels, consistent with previous broad sense heritability estimates. The effect of sex on gene expression levels was most noticeable on the X chromosome, which contained 15 of the 20 significantly differentially expressed genes. A high concordance was observed between the sex difference test statistics and surveys of genes escaping X chromosome inactivation. Notably, several autosomal genes showed significant differences in gene expression between the sexes despite much of the cellular environment differences being effectively removed in the cell lines. A publicly available gene expression data set from the CEPH families was used to validate the results. The heritability of gene expression levels as estimated from the two data sets showed a highly significant positive correlation, particularly when both estimates were close to one and thus had the smallest standard error. There was a large concordance between the genes significantly differentially expressed between the sexes in the two data sets. Analysis of the variability of probe binding intensities within a probe set indicated that results are robust to the possible presence of polymorphisms in the target sequences. |
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DATE PUBLISHED |
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HISTORY |
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PUBSTATUS |
PUBSTATUSDATE |
aheadofprint |
2006/12/12 |
pubmed |
2006/12/14 09:00 |
medline |
2007/04/27 09:00 |
entrez |
2006/12/14 09:00 |
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AUTHORS |
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NAME |
COLLECTIVENAME |
LASTNAME |
FORENAME |
INITIALS |
AFFILIATION |
AFFILIATIONINFO |
McRae AF |
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McRae |
Allan F |
AF |
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Genetic Epidemiology Group, Queensland Institute of Medical Research, Herston, QLD 4029, Australia. allan.mcrae@qimr.edu.au |
Matigian NA |
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Matigian |
Nicholas A |
NA |
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Vadlamudi L |
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Vadlamudi |
Lata |
L |
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Mulley JC |
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Mulley |
John C |
JC |
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Mowry B |
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Mowry |
Bryan |
B |
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Martin NG |
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Martin |
Nicholas G |
NG |
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Berkovic SF |
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Berkovic |
Sam F |
SF |
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Hayward NK |
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Hayward |
Nicholas K |
NK |
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Visscher PM |
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Visscher |
Peter M |
PM |
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INVESTIGATORS |
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JOURNAL |
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VOLUME: 16 |
ISSUE: 4 |
TITLE: Human molecular genetics |
ISOABBREVIATION: Hum. Mol. Genet. |
YEAR: 2007 |
MONTH: Feb |
DAY: 15 |
MEDLINEDATE: |
SEASON: |
CITEDMEDIUM: Print |
ISSN: 0964-6906 |
ISSNTYPE: Print |
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MEDLINE JOURNAL |
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MEDLINETA: Hum Mol Genet |
COUNTRY: England |
ISSNLINKING: 0964-6906 |
NLMUNIQUEID: 9208958 |
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PUBLICATION TYPE |
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PUBLICATIONTYPE TEXT |
Journal Article |
Research Support, Non-U.S. Gov't |
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COMMENTS AND CORRECTIONS |
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GRANTS |
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GENERAL NOTE |
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KEYWORDS |
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MESH HEADINGS |
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DESCRIPTORNAME |
QUALIFIERNAME |
Adult |
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Cell Line, Transformed |
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Cell Transformation, Viral |
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Databases, Factual |
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Family |
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Female |
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Gene Expression Regulation |
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Genotype |
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Humans |
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Lymphocytes |
metabolism |
Male |
metabolism |
Middle Aged |
metabolism |
Oligonucleotide Array Sequence Analysis |
metabolism |
Sex Characteristics |
metabolism |
Twins, Monozygotic |
metabolism |
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SUPPLEMENTARY MESH |
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GENE SYMBOLS |
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CHEMICALS |
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OTHER ID's |
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