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| PMID |
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| TITLE |
|
| Replicated effects of sex and genotype on gene expression in human lymphoblastoid cell lines. |
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| ABSTRACT |
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| The expression level for 15,887 transcripts in lymphoblastoid cell lines from 19 monozygotic twin pairs (10 male, 9 female) were analysed for the effects of genotype and sex. On an average, the effect of twin pairs explained 31% of the variance in normalized gene expression levels, consistent with previous broad sense heritability estimates. The effect of sex on gene expression levels was most noticeable on the X chromosome, which contained 15 of the 20 significantly differentially expressed genes. A high concordance was observed between the sex difference test statistics and surveys of genes escaping X chromosome inactivation. Notably, several autosomal genes showed significant differences in gene expression between the sexes despite much of the cellular environment differences being effectively removed in the cell lines. A publicly available gene expression data set from the CEPH families was used to validate the results. The heritability of gene expression levels as estimated from the two data sets showed a highly significant positive correlation, particularly when both estimates were close to one and thus had the smallest standard error. There was a large concordance between the genes significantly differentially expressed between the sexes in the two data sets. Analysis of the variability of probe binding intensities within a probe set indicated that results are robust to the possible presence of polymorphisms in the target sequences. |
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| DATE PUBLISHED |
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| HISTORY |
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| PUBSTATUS |
PUBSTATUSDATE |
| aheadofprint |
2006/12/12 |
| pubmed |
2006/12/14 09:00 |
| medline |
2007/04/27 09:00 |
| entrez |
2006/12/14 09:00 |
|
| AUTHORS |
|
| NAME |
COLLECTIVENAME |
LASTNAME |
FORENAME |
INITIALS |
AFFILIATION |
AFFILIATIONINFO |
| McRae AF |
|
McRae |
Allan F |
AF |
|
Genetic Epidemiology Group, Queensland Institute of Medical Research, Herston, QLD 4029, Australia. allan.mcrae@qimr.edu.au |
| Matigian NA |
|
Matigian |
Nicholas A |
NA |
|
|
| Vadlamudi L |
|
Vadlamudi |
Lata |
L |
|
|
| Mulley JC |
|
Mulley |
John C |
JC |
|
|
| Mowry B |
|
Mowry |
Bryan |
B |
|
|
| Martin NG |
|
Martin |
Nicholas G |
NG |
|
|
| Berkovic SF |
|
Berkovic |
Sam F |
SF |
|
|
| Hayward NK |
|
Hayward |
Nicholas K |
NK |
|
|
| Visscher PM |
|
Visscher |
Peter M |
PM |
|
|
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| INVESTIGATORS |
|
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| JOURNAL |
|
| VOLUME: 16 |
| ISSUE: 4 |
| TITLE: Human molecular genetics |
| ISOABBREVIATION: Hum. Mol. Genet. |
| YEAR: 2007 |
| MONTH: Feb |
| DAY: 15 |
| MEDLINEDATE: |
| SEASON: |
| CITEDMEDIUM: Print |
| ISSN: 0964-6906 |
| ISSNTYPE: Print |
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| MEDLINE JOURNAL |
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| MEDLINETA: Hum Mol Genet |
| COUNTRY: England |
| ISSNLINKING: 0964-6906 |
| NLMUNIQUEID: 9208958 |
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| PUBLICATION TYPE |
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| PUBLICATIONTYPE TEXT |
| Journal Article |
| Research Support, Non-U.S. Gov't |
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| COMMENTS AND CORRECTIONS |
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| GRANTS |
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| GENERAL NOTE |
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| KEYWORDS |
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| MESH HEADINGS |
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| DESCRIPTORNAME |
QUALIFIERNAME |
| Adult |
|
| Cell Line, Transformed |
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| Cell Transformation, Viral |
|
| Databases, Factual |
|
| Family |
|
| Female |
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| Gene Expression Regulation |
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| Genotype |
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| Humans |
|
| Lymphocytes |
metabolism |
| Male |
metabolism |
| Middle Aged |
metabolism |
| Oligonucleotide Array Sequence Analysis |
metabolism |
| Sex Characteristics |
metabolism |
| Twins, Monozygotic |
metabolism |
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| SUPPLEMENTARY MESH |
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| GENE SYMBOLS |
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| CHEMICALS |
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| OTHER ID's |
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